anti human mouse cd11b apc cy7  (Cytek Biosciences)

Journal: bioRxiv

Article Title: Assessing a Role for Sarcospan in Immune Function

doi: 10.1101/2024.03.31.587501

Figure Lengend Snippet: Evaluating Roles for Sarcospan in Immune Cell Function and Hematopoietic Cell Lineages. (A) Immune-related functional relationship networks were examined using ImmuNet and shown for the SSPN protein in the hematopoietic cell lineage dataset at a confidence level > 0.8949. (B) The table shows the eleven strongest functional relationships between hematopoietic cell lineages and SSPN. In (A) extracellular flow cytometry was utilized to identify SSPN-expressing immune cells in WT C57BL6/J mice. SSPN was detected on unstimulated B cells, CD11b+, and CD11c+ cells while unstimulated granulocytes and T cells had either no or low SSPN expression. In (B) B cells obtained from WT male and female mice exhibit similar SSPN expression profiles. In (C) RNA-seq data (nTPM) is plotted from the Human Protein Atlas for different B cell types (naïve, non-switched memory, switched memory, plasmablast, exhausted memory) (Monaco et al. 2019).

Article Snippet: Cells were incubated with anti-mouse primary antibodies to assess immune populations with the following conjugated antibodies: B220-APC (Tonbo Biosciences, San Diego, CA; clone RA3-6B2), CD19-PE/Dazzle594 (BioLegend, San Diego, CA; clone 6D5), CD3-AlexaFluor488 (BioLegend, clone 17A2), CD4-violetFluor450 (Tonbo Biosciences, clone GK1.5), CD8-PE/Cy7 (Tonbo Biosciences, clone 53-6.7), NK1.1-PE (Tonbo Biosciences, clone PK136), CD335/NKp46-APC (BioLegend, clone 29A1.4), CD11b-APC/Cy7 (Tonbo Biosciences, clone M1/70), CD11c-PerCp/Cy5.5 (Tonbo Biosciences, clone N418), Ly6G-PE (Tonbo Biosciences, clone RB6-8C5), and F4/80-FITC (Tonbo Biosciences, clone BM8.1,).

Techniques: Cell Function Assay, Functional Assay, Flow Cytometry, Expressing, RNA Sequencing

Journal: iScience

Article Title: Engraftment of adult hematopoietic stem and progenitor cells in a novel model of humanized mice

doi: 10.1016/j.isci.2024.109238

Figure Lengend Snippet:

Article Snippet: Mouse CD11b-APC/Cy7 (M1/70) , TONBO , 25-0112-U100; RRID: AB_3094465.

Techniques: Recombinant, Staining, Red Blood Cell Lysis, Enzyme-linked Immunosorbent Assay, Software

Journal: PLoS ONE

Article Title: Microparticles Engineered to Highly Express Peroxisome Proliferator-Activated Receptor-γ Decreased Inflammatory Mediator Production and Increased Adhesion of Recipient Monocytes

doi: 10.1371/journal.pone.0113189

Figure Lengend Snippet: PBMCs were isolated and cultured for 24 hours with green fluorescent protein (GFP)+ microparticles, and then washed, stained, and analyzed by flow cytometry. A , Cells that internalize or bind to GFP+ microparticles (right) exhibit green fluorescence compared to cells not treated with microparticles (left). B , Cells were gated on GFP and then subgated on CD11b, followed by CD3 and CD19 to identify specific cell types. C , PBMCs from three representative donors demonstrate that the majority of the GFP positive cells are positive for CD11b (white boxes). D , Ten times more microparticles were added to PBMCs (High MP∶target) to identify the any other subpopulation to internalize microparticles. One representative donor PBMC demonstrates that CD3+ cells (grey boxes) were the second cell type to internalize microparticles according to percentage (left) and numbers (right) of GFP-positive PBMCs.

Article Snippet: Antibodies used in flow cytometry analysis were: biotin mouse anti-human CD49e (555616, BD Biosciences) with streptavidin-APC (SA1005, Caltag, Buckingham, MK18 1TF), alexa fluor 647 mouse anti-fibronectin (563098, BD Biosciences), alexa fluor 700 mouse anti-human CD19 (557921, BD Biosciences), APC-Cy7 mouse anti-human CD11b (560914 BD Biosciences), PE mouse anti-human CD3 (9515-09, Southern Biotechnology, Birmingham, AL), APC mouse anti-human CD36 (561822, BD Biosciences).

Techniques: Isolation, Cell Culture, Staining, Flow Cytometry, Fluorescence